Journal: Cell Biology and Toxicology

Article Title: BMSC-derived extracellular vesicles affect gluconeogenesis and lipogenesis by releasing 5'-tRF-GlyCCC to improve MAFLD insulin sensitivity

doi: 10.1007/s10565-025-10132-5

Figure Lengend Snippet: 5'-tRF-GlyCCC may bind to FoxO3 3′UTR to regulate glucose production. A The binding sites of in FoxO3 3′-UTR. B The transcriptional activity of FoxO3 regulated by 5'-tRF-GlyCCC was detected by dual-luciferase reporter gene assay. C The protein expressions of p-FoxO3, FoxO3, G6Pase, PEPCK were detected by western blotting. D The quantification of WB data were normalized to GAPDH and expressed as relative value. E The quantitative detection of glucose production through ELISA kit. ** p < 0.01, *** p <0.001, **** p <0.0001.

Article Snippet: The experiment involved conjugating the samples with normal IgG (produced by Cell Signaling Technology in the USA) or an anti-FoxO3 antibody (also obtained from Cell Signaling Technology in the USA) at a temperature of 4 °C for an extended period overnight.

Techniques: Binding Assay, Activity Assay, Luciferase, Reporter Gene Assay, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Cell Biology and Toxicology

Article Title: BMSC-derived extracellular vesicles affect gluconeogenesis and lipogenesis by releasing 5'-tRF-GlyCCC to improve MAFLD insulin sensitivity

doi: 10.1007/s10565-025-10132-5

Figure Lengend Snippet: 5'-tRF-GlyCCC mediates glucose production by downregulating the expression of FoxO3. A The protein expression of FoxO3, G6Pase, and PEPCK in different groups of HepG2 cells was detected by WB. B The quantification of WB data were normalized to GAPDH. C The quantitative detection of glucose production in different groups of HepG2 cells was detected ELISA kit. D Schematic diagram of the identified binding sequence in the promoter region of the PEPCK gene targeted by FoxO3. E The occupancy of FoxO3 on PEPCK promoter in PA-treated HepG2 cells was detected using ChIP assay. ** p < 0.01, *** p <0.001.

Article Snippet: The experiment involved conjugating the samples with normal IgG (produced by Cell Signaling Technology in the USA) or an anti-FoxO3 antibody (also obtained from Cell Signaling Technology in the USA) at a temperature of 4 °C for an extended period overnight.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Binding Assay, Sequencing